Scientists Explain Why Senior Citizens Lose Energy; Hope to Slow Aging Process
Discovered major declines in enzyme known as the Lon protease, as human cells grow older and body fights oxidative damage
Aug. 31, 2011 What senior citizen has not wondered why their energy declines as they age? In a new study, scientists
think they may have found the answer to why humans lose energy with age and hope this will point the way to new diets or pharmaceuticals to
slow the aging process.
When the body fights oxidative damage, it calls up a reservist enzyme that protects cells but only if those cells are
relatively young, a study has found.
Biologists at the University of Southern California discovered major declines in the availability of an enzyme, known as
the Lon protease, as human cells grow older.
The researchers showed that when oxidative agents attack the power centers of young cells, the cells respond by calling
up reinforcements of the enzyme, which breaks up and removes damaged proteins.
As the cells age, they lose the ability to mobilize large numbers of Lon, the researchers reported in The Journals of
Senior author Kelvin J. A. Davies, a professor at the USC Leonard Davis School of Gerontology, used a war analogy to
explain that no "standing army" of Lon protease can endure an attack by invading oxidants without calling up reserves.
"Once the war has started, what's your capacity to keep producing to protect your vital resources and keep the fight
going?" he asked.
Since aging is the longest war, the USC study suggests a more important role for the reservist enzyme than previously
Lon protects the mitochondria tiny organisms in the cell that convert oxygen into energy. The conversion is never
perfect: Some oxygen leaks and combines with other elements to create damaging oxidants.
Oxidation is the process behind rust and food spoilage. In the body, oxidation can damage or destroy almost any tissue.
Lon removes oxidized proteins from the mitochondria and also plays a vital role in helping to make new mitochondria.
"We know that mitochondrial function declines with age, which is a major limitation to cells. One of the components of
that decline is the loss of Lon. The ability of Lon to be induced by [oxidative] stress is a very important component of overall stress
resistance," Davies said.
Davies and his team worked with a line of human lung cells. They exposed the cells to hydrogen peroxide, a powerful
oxidant that is a byproduct of energy production and that also can result from metabolism of some drugs, toxins, pesticides and herbicides.
To fight the oxidant, young cells doubled the size of their Lon army within five hours and maintained it for a day. In
some experiments, young cells increased their Lon army as much as seven-fold.
Middle-aged cells took a full day to double their Lon army, during which time the cells were exposed to harmful levels of
Older cells started with a standing Lon army only half as large and showed no statistically significant increase in Lon
levels over 24 hours.
The Davies group, which discovered Lon in 2002, previously had shown that Lon's standing army gets smaller with age and
that the anti-oxidant power of Lon depends more on its reserves than on enzymes present when stress first hits the body.
The latest study completes the picture of Lon's sluggish response as senescent cells a technical term for cells that
mimic several key features of the aging process try to cope with stress.
"In the senescent cells, the Lon levels are drastically low to begin with, and they don't increase" in response to
stress, Davies said.
Scientists have known for decades that mitochondria become less efficient with age, contributing to the body's loss of
"It may well be that our ability to induce Lon synthesis and our loss of adaptability to stress may be an even more
significant factor in the aging process," Davies said.
Davies and others are investigating potential treatments to boost the function of Lon. Costly enzyme supplements are
useless, Davies noted, since the digestive system breaks down the enzyme to amino acids before it can reach its target.
"It's a lot cheaper to buy a piece of meat and get the same amino acids," he said.
Davies holds the James E. Birren Chair in Gerontology, with a joint appointment in molecular biology at the USC Dornsife
College of Letters, Arts and Sciences.
His co-authors were USC postdoctoral fellow Jenny Ngo, undergraduate students Laura Pomatto and Alison Koop, and former
graduate student Daniela Bota, now an assistant professor at the University of California, Irvine Medical Center.
Funding for the research came from the National Institute of Environmental Health Sciences, part of the National
Institutes of Health.
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